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PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)

Key features and details

  • PerCP/Cy5.5® Rat monoclonal [M5/114.15.2] to H2-Ea
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Isotype: IgG2b

Overview

  • Product name

    PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2]
    See all H2-Ea primary antibodies

  • Description

    PerCP/Cy5.5® Rat monoclonal [M5/114.15.2] to H2-Ea

  • Host species

    Rat

  • Conjugation

    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm

  • Specificity

    ab218783 reacts with Mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. It does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7.

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • C57Bl/6 splenocytes.

  • General notes

     This product was previously labelled as MHC Class II IA + IE

     

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Store In the Dark.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.87% Sodium chloride, 0.1% Gelatin, 0.12% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab218783 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

  • Clonality

    Monoclonal

  • Clone number

    M5/114.15.2

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)
    Flow Cytometry - PerCP/Cy5.5® Anti-H2-Ea antibody [M5/114.15.2] (ab218783)

    Flow cytometric analysis of C57Bl/6 splenocytes labeling H2-Ea with 0.25µg ab218783 (solid line), compared with 0.25µg PerCP/Cy5.5® Rat IgG2b isotype control (dashed line).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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