Pembrolizumab ELISA Kit (Keytruda®) (ab237652)
Key features and details
- Sensitivity: 10 ng/ml
- Range: 30 ng/ml - 1000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Overview
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Product name
Pembrolizumab ELISA Kit (Keytruda®) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Quantitative -
Sensitivity
10 ng/ml -
Range
30 ng/ml - 1000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Pembrolizumab ELISA Kit (Keytruda®) (ab237652) is a highly specific and sensitive kit designed for the in vitro determination of Pembrolizumab in human serum and plasma. The density of color is proportional to the amount of Pembrolizumab captured from the samples and can be quantified when compared with standard curve.
Pembrolizumab (Keytruda®) is an antibody drug that targets the cell surface receptor programmed cell death protein 1 (PD-1) found on T cells. By preventing the binding of its ligands (PD-L1 and PD-L2), Pembrolizumab induces an anti-tumor immune response. Upregulation of PD-1 ligands is a mechanism for tumors to evade anti-tumor immune response; when PD-1 binds its ligand, the T cell receives an inhibitory signal leading to T cell anergy and blockade of anti-tumour immune response. Instead of directly targeting tumor tissue to induce tumor cell death, Pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Assay Buffer 2 x 50ml HRP-conjugate Probe 1 x 12ml Micro ELISA Plate 1 unit Pembrolizumab Standard S1 1 x 0.3ml Pembrolizumab Standard S2 1 x 0.3ml Pembrolizumab Standard S3 1 x 0.3ml Pembrolizumab Standard S4 1 x 0.3ml Pembrolizumab Standard S5 1 x 0.3ml Pembrolizumab Standard S6 1 x 0.3ml Pembrolizumab Standard S7 1 x 0.3ml Plate sealers 2 units Stop Solution 1 x 12ml TMB substrate 1 x 12ml Wash buffer (20X) 1 x 50ml -
Relevance
Pembrolizumab (Keytruda®) is an antibody drug that targets the cell surface receptor programmed cell death protein 1 (PD-1) found on T cells. By preventing the binding of its ligands (PD-L1 and PD-L2), pembrolizumab induces an antitumor immune response. Upregulation of PD-1 ligands is a mechanism for tumors to evade antitumor immune response; when PD-1 binds its ligand, the T cell receives an inhibitory signal leading to T cell anergy and blockade of anti-tumour immune response. Instead of directly targeting tumor tissue to induce tumor cell death, pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells. -
Alternative names
- Keytruda
Images
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Example of Pembrolizumab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.