PE Anti-ICOS antibody [EPR22181] (ab245909)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR22181] to ICOS
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Overview
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Product name
PE Anti-ICOS antibody [EPR22181]
See all ICOS primary antibodies -
Description
PE Rabbit monoclonal [EPR22181] to ICOS -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: 293T transfected with GFP-tagged ICOS overexpression vector.ICC/IF: 293T transfected with GFP-tagged ICOS.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22181 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence analysis of 4% PFA-fixed, 0.1% Triton X-100-permeabilized 293T (human embryonic kidney epithelial cell) cells using ab245908 at 1/200 dilution (2.5 µg/ml) (red). Counterstain: DAPI (blue).
Confocal image showing increased cytoplasmic staining in 293T cells transfected with ICOS with GFP tag.
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Flow cytometric analysis of 293T transfected with GFP-tagged ICOS overexpression vector using ab209478 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Phycoerythrin) (Left panel) and ab245909 at 1/500 dilution (Right panel).
Blocking: 10% Goat serum.
Gated on viable cells.
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