Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3618] to CD79a
Suitable for: ICC/IF, WB, IHC-P
Reacts with: Human

Product name

Anti-CD79a antibody [EP3618]
See all CD79a primary antibodies
Description

Rabbit monoclonal [EP3618] to CD79a
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD79a aa 50-150 (extracellular). The exact sequence is proprietary.
Positive control
- Raji cell, Daudi cell, human tonsil tissue and human spleen tissue lysates. Raji cells. Human tonsil tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.16 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP3618
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 at 1/100 dilution staining CD79a in paraffin-embedded human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-CD79a antibody [EP3618] (ab79414)

All lanes : Anti-CD79a antibody [EP3618] (ab79414) at 1/10000 dilution

Lane 1 : Raji cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Human tonsil tissue lysate
Lane 4 : Human spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 25 kDa
Observed band size: 44-48 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-CD79a antibody [EP3618] (ab79414)

Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) cells labelling CD79a with purified ab79414 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal spleen tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal brain tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal colon tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal heart tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal brain tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing negative staining in Normal heart tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD79a antibody [EP3618] (ab79414)

ab79414 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD79a antibody [EP3618] (ab79414)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CD79a antibody [EP3618] (ab79414)

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