Vector contains genes for bacterial and plant selection with kanamycin and the gusA reporter gene.

The pCambia2301 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with kanamycin
Simple means to construct translational fusions to gusA reporter genes

Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV₃₅S promoter and terminated by the CaMV₃₅S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.

This vector contains a fully functional gusA reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses E.coli gusA with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual A.tumefaciens cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the gusA gene and insert their own gene of interest in its place or use this vector to create fusions of gusA with their gene of interest. This vector contains the pUC18 polylinker-lacZa.

Genbank Sequence data

Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (aphIII/nptIII/3’5’’-aminoglycoside phosphotransferase typeIII from
Enterococcus faecalis pJH1) in bacteria: use 50 μg/mL in E.coli and A.tumefaciens.

In plant system: