pCambia1390 Plant Expression Vector (ab275767)
Overview
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Product name
pCambia1390 Plant Expression Vector -
General notes
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system.
The pCambia1304 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with hygromycin B
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal.This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes. Contains the simpler pUC9 polylinker which does not contain potentially confounding start or stop codons.
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (aphIII/nptIII/3’5’’-aminoglycoside phosphotransferase typeIII from Enterococcus faecalis pJH1) in bacteria: use 50 μg/mL in E.coli and A.tumefaciens.In plant system:
Resistance Gene hph (From a Klebsiella species, via E.coli) Transformed plant selection antibiotic Hygromycin B Concentration in Arabidopsis 50 μg/mL in germination medium Concentration in Rice 50 μg/mL in 2N6 then in RGH6 Concentration in Tobacco 50 μg/mL in RMOP then in MST
Properties
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Form
Lyophilized -
Storage instructions
Shipped at 4°C. Store at -20°C. -
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Images
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pCambia1390 Plant Expression Vector (ab275767)