pCambia1304 Plant Expression Vector (ab275550)
Overview
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Product name
pCambia1304 Plant Expression Vector -
General notes
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and the mgfp5:gusA fusion reporter genes.
The pCambia1304 vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with hygromycin B
Simple means to construct translational fusions to mgfp5-gusA reporter genesPlant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.
This vector contains the mgfp5 version of the Aequoria victoria green fluorescent protein in translational fusion with gusA.
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (aphIII/nptIII/3’5’’-aminoglycoside phosphotransferase typeIII from Enterococcus faecalis pJH1) in bacteria: use 50 μg/mL in E.coli and A.tumefaciens.In plant system:
Resistance Gene hph (From a Klebsiella species, via E.coli) Transformed plant selection antibiotic Hygromycin B Concentration in Arabidopsis 50 μg/mL in germination medium Concentration in Rice 50 μg/mL in 2N6 then in RGH6 Concentration in Tobacco 50 μg/mL in RMOP then in MST
Properties
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Form
Lyophilized -
Storage instructions
Shipped at 4°C. Store at -20°C. -
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Images
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pCambia1304 Plant Expression Vector (ab275550)