Vector contains chloramphenicol resistance for bacterial selection, hygromycin B resistance for plant selection and the gusA fusion reporter gene and lacZ for white/blue selection.

The pCambia1281Z vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with chloramphenicol
Plant selection with hygromycin B
Simple means to construct translational fusions to gusA reporter genes

Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV₃₅S promoter and terminated by the CaMV₃₅S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.

Designed for promoter testing in planta, this vector features a promoterless version of gusA (N358Q) with the catalase intron immediately downstream of a truncated lacZa containing the pUC8 polylinker. The truncated lacZa is functional for blue/white screening of clones in suitable E.coli host strains.

Genbank Sequence data

Concentration of Antibiotics for selection.
In bacterial system:
Chloramphenicol resistance (cat – chloramphenicol acetyltransferase from E.coli Tn-cam204) in bacteria: use 10μg/mL in E.coli and A.tumefaciens.

In plant system: