pCambia1281Z Plant Expression Vector (ab275779)
Overview
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Product name
pCambia1281Z Plant Expression Vector -
General notes
Vector contains chloramphenicol resistance for bacterial selection, hygromycin B resistance for plant selection and the gusA fusion reporter gene and lacZ for white/blue selection.
The pCambia1281Z vector offers:
High copy number in E.coli for high DNA yields
pVS1 replicon for high stability in Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with chloramphenicol
Plant selection with hygromycin B
Simple means to construct translational fusions to gusA reporter genesPlant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilized metal affinity chromatography resins.
Designed for promoter testing in planta, this vector features a promoterless version of gusA (N358Q) with the catalase intron immediately downstream of a truncated lacZa containing the pUC8 polylinker. The truncated lacZa is functional for blue/white screening of clones in suitable E.coli host strains.
Concentration of Antibiotics for selection.
In bacterial system:
Chloramphenicol resistance (cat – chloramphenicol acetyltransferase from E.coli Tn-cam204) in bacteria: use 10μg/mL in E.coli and A.tumefaciens.In plant system:
Resistance Gene hph (From a Klebsiella species, via E.coli) Transformed plant selection antibiotic Hygromycin B Concentration in Arabidopsis 50 μg/mL in germination medium Concentration in Rice 50 μg/mL in 2N6 then in RGH6 Concentration in Tobacco 50 μg/mL in RMOP then in MST
Properties
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Form
Lyophilized -
Storage instructions
Shipped at 4°C. Store at -20°C. -
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Images
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pCambia1281Z Plant Expression Vector (ab275779)
