Oxaloacetate Assay Kit (ab83428)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 2 µM
Overview
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Product name
Oxaloacetate Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 2 µM -
Range
2 µM - 200 µM -
Assay time
0h 40m -
Product overview
Abcam's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (?max= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
Visit our FAQs page for tips and troubleshooting. -
Notes
Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Developer Green 1 vial OAA Assay Buffer WM 1 x 25ml OAA Enzyme Mix Purple 1 vial OAA Probe (in DMSO) Red 1 x 200µl OAA Standard (10 µmol) Yellow 1 vial -
Research areas
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Relevance
Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. -
Alternative names
- OAA
- Oxaloacetic acid
Images
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Functional Studies- Oxaloacetate (OAA) Assay Kit (ab83428)
Oxaloacetate fluorimetrically measured in biofluids (after spin column) showing concentration (micromolar)
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Functional Studies- Oxaloacetate (OAA) Assay Kit (ab83428)Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted
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Functional Studies - Oxaloacetate (OAA) Assay Kit (ab83428)Oxaloacetate standard curve generated following the kit protocol.