Omalizumab ELISA Kit (ab237650)
Key features and details
- Sensitivity: 10 ng/ml
- Range: 30 ng/ml - 1000 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Omalizumab ELISA Kit
See all Omalizumab kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10 ng/ml -
Range
30 ng/ml - 1000 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Omalizumab ELISA Kit (ab237650) has been developed for specific quantification of Omalizumab concentration in human serum or plasma with high sensitivity and reproducibility. The density of color is proportional to the amount of Omalizumab captured from the samples and can be quantified when compared with standard curve.
Omalizumab is a recombinant DNA-derived humanized IgG1k monoclonal antibody that selectively binds to human immunoglobulin E (IgE). Omalizumab inhibits the binding of IgE to the high-affinity IgE receptor (FceRI) on the surface of mast cells and basophils. Reduction in surface-bound IgE on FceRI-bearing cells limits the degree of release of mediators of the allergic response. Omalizumab is used to treat severe, persistent asthma.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests Micro ELISA Plate 1 unit Omalizumab Standard S1 1 x 1ml Omalizumab Standard S2 1 x 1ml Omalizumab Standard S3 1 x 1ml Omalizumab Standard S4 1 x 1ml Omalizumab Standard S5 1 x 1ml Omalizumab Standard S6 1 x 1ml Omalizumab Standard S7 1 x 1ml Assay Buffer 1 x 50ml HRP-conjugate Probe 1 x 12ml TMB substrate 1 x 12ml Stop Solution 1 x 12ml Wash buffer (20X) 1 x 50ml Plate sealers 2 units -
Relevance
Omalizumab (Xolair®) is a recombinant DNA-derived humanized IgG1k monoclonal antibody that selectively binds to human immunoglobulin E (IgE). Xolair inhibits the binding of IgE to the high-affinity IgE receptor (FceRI) on the surface of mast cells and basophils. Reduction in surface-bound IgE on FceRI-bearing cells limits the degree of release of mediators of the allergic response. Xolair is used to treat severe, persistent asthma. -
Alternative names
- Xolair
Images
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Example of Omalizumab standard curve
Typical Standard Curve: This standard curve is for demonstration only. A standard curve must be run with each assay.