Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22070] to IL-4I1/LAO
Suitable for: IP, WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-IL-4I1/LAO antibody [EPR22070]
See all IL-4I1/LAO primary antibodies
Description

Rabbit monoclonal [EPR22070] to IL-4I1/LAO
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate . IHC-P: Human tonsil, Hodgkin's lymphoma and diffuse large B-cell lymphoma tissue. ICC/IF: HEK-293 whole cell lysate. Flow: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag. IP: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22070
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

Immunohistochemical analysis of paraffin-embedded human difuse large B-cell lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human diffuse large B-cell lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in human Hodgkin's lymphoma (PMID: 19436310) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue stained for IL-4I1/LAO with ab222102 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Granular cytoplasmic staining in macrophages of human tonsil (PMID: 17356132; PMID: 19436310) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

IL-4I1/LAO was immunoprecipitated from 0.35mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate with ab222102 at 1/30 dilution. Western blot was perfromed from the immunoprecipitate using ab222102 at 1/1000 dilution, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate 10μg (Input).

Lane 2: ab222102 IP in HEK-293 transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (+).

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222102 in HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag whole cell lysate (-).

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 seconds.
Flow Cytometry - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag labeling IL-4I1/LAO with ab222102 at 1/500 dilution (Right) compared to Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilzed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling IL-4I1/LAO (white) with ab222102 at 1/50 dilution, followed by ab150079 AlexaFluor^®647 Goat anti-Rabbit secondary at 1/1000 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged IL-4I1/LAO expression vector. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150079 AlexaFluor^®647 Goat anti-Rabbit secondary at 1/1000 dilution.
Western blot - Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

All lanes : Anti-IL-4I1/LAO antibody [EPR22070] (ab222102) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-GFP-tag, whole cell lysate
Lane 2 : HEK-293T transfected with IL-4I1/LAO expression vector containing a myc-GFP-tag, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 63 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Exposure time: 6 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-IL-4I1/LAO antibody [EPR22070] (ab222102)

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