Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter (ab139474)
Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence | Cytopainter (ab139474) contains two proprietary dyes suitable for simultaneous live-cell staining of nucleoli and nuclei. The dyes allow examination of nucleolar dynamic changes in intracellular distribution, trafficking and localization arising from biological processes such as the cell cycle and ribosome biogenesis. The kit is compatible with most fluorescence detection systems, including conventional and confocal fluorescence microscopes, as well as High Content Screening (HCS) platforms.
This kit is specifically designed for visualizing nucleoli and nuclei in living cells. A control nucleolus perturbation agent, actinomycin D, is provided for monitoring changes in nucleolar dynamics. Potential applications for this kit include monitoring impaired ribosome biogenesis, inhibition transcription, cell cycle dynamics and cellular stress, as well as the distribution, trafficking and dynamics of nucleolar proteins, the distribution of viral proteins, and potentially as an aid in identifying cancer cells.
Review other dyes and kits for nucleolar staining, or the live cell staining fluorescent dyes guide
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 500 tests 10X Assay Buffer 1 x 15ml Actinomycin D Control 1 x 125µg Green Detection Reagent 1 x 50µl Red Detection Reagent 1 x 50µl -
Research areas
Images
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Absorption and fluorescence emission spectra for ab139474Absorption and fluorescence emission spectra for ab139474 Green (A) and Red (B) dyes. All spectra were determined in 1X Assay Buffer.
