Astana Biomed Group, an authorized Abcam distributor in Central Asia

Native human Cathepsin D protein (ab91123)

Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE, WB

Description

  • Product name

    Native human Cathepsin D protein
    See all Cathepsin D proteins and peptides

  • Biological activity

    Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native

  • Accession

    P07339

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Human

    • Sequence

      MQPSSLLPLALCLLAAPASALVRIPLHKFTSIRRTMSEVGGSVEDLIAKG PVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGS SNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGY LSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILG MAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGT DSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSL MVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLS PEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRD NNRVGFAEAARL

    • Additional sequence information

      Source: Human Plasma

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

    pH: 6.50
    Constituent: 0.0328% Sodium phosphate

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Resuspend a 25ug vial with 71.4uL of deionized H2O to give a protein concentration of 0.350mg/mL, which is the original protein concentration of the lot when it was finished. This should then be aliquotted into suitable amounts in a screw top vial to prevent sublimation and stored at

    General Info

    • Alternative names

      • CatD
      • CATD_HUMAN
      • Cathepsin D
      • Cathepsin D heavy chain
      • CD
      • Ceroid lipofuscinosis neuronal 10
      • CLN10
      • CPSD
      • ctsd
      • Epididymis secretory sperm binding protein Li 130P
      • HEL S 130P
      • Lysosomal aspartyl peptidase
      • Lysosomal aspartyl protease
      • MGC2311
      see all

    • Function

      Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

    • Tissue specificity

      Expressed in the aorta extrcellular space (at protein level).

    • Involvement in disease

      Ceroid lipofuscinosis, neuronal, 10

    • Sequence similarities

      Belongs to the peptidase A1 family.
      Contains 1 peptidase A1 domain.

    • Post-translational
      modifications

      N- and O-glycosylated.

    • Cellular localization

      Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
    • Information by UniProt

    Images

    • SDS-PAGE - Native human Cathepsin D protein (ab91123)
      SDS-PAGE - Native human Cathepsin D protein (ab91123)
      SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
      Lane 1. Molecular weight markers
      Lane 2. 4 μg Cathepsin D (non-reduced/no heat)
      Lane 3. 7 μg Cathepsin D (non-reduced/no heat)
      Lane 4. Molecular weight markers
      Lane 5. 4 μg Cathepsin D (reduced/heated)
      Lane 6. 7 μg Cathepsin D (reduced/heated)
    • Western blot - Native human Cathepsin D protein (ab91123)
      Western blot - Native human Cathepsin D protein (ab91123)
      Anti-Cathepsin D antibody [4G2] (ab40697) at 1/500 dilution + Native human Cathepsin D protein (ab91123) at 0.1 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 30 seconds

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

References (1)

Publishing research using ab91123? Please let us know so that we can cite the reference in this datasheet.

ab91123 has been referenced in 1 publication.

  • Brings S  et al. Urinary cathepsin L is predictive of changes in albuminuria and correlates with glucosepane in patients with type 2 diabetes in a closed-cohort study. J Diabetes Complications N/A:107648 (2020). PubMed: 32532588

Images

  • SDS-PAGE - Native human Cathepsin D protein (ab91123)
    SDS-PAGE - Native human Cathepsin D protein (ab91123)
    SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1. Molecular weight markers
    Lane 2. 4 μg Cathepsin D (non-reduced/no heat)
    Lane 3. 7 μg Cathepsin D (non-reduced/no heat)
    Lane 4. Molecular weight markers
    Lane 5. 4 μg Cathepsin D (reduced/heated)
    Lane 6. 7 μg Cathepsin D (reduced/heated)
  • Western blot - Native human Cathepsin D protein (ab91123)
    Western blot - Native human Cathepsin D protein (ab91123)
    Anti-Cathepsin D antibody [4G2] (ab40697) at 1/500 dilution + Native human Cathepsin D protein (ab91123) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 30 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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