NADP Regeneration Kit (ab228548)
Overview
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Product name
NADP Regeneration Kit
See all NADP/NADPH kits -
Product overview
NADP Regeneration Kit (ab228548) provides two ready to use solutions to regenerate NADP by a simple mixing. This kit can be used for all NADP-requiring systems (e.g. 12-Ketochenodeoxycholic Acid synthesis, Ferredoxin Reductase System). About 300-500 enzyme assays can be performed using this kit. The total number of assays that can be performed depends on the experimental design.
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Notes
NADP is the electron acceptor for biosynthetic reactions and dehydrogenation reactions. NADP is used for anabolic pathways, such as lipid synthesis, cholesterol synthesis and fatty acid chain elongation. It is also a necessary cofactor in many xenobiotic metabolism reactions. In chloroplasts, NADP is reduced to NADPH by ferredoxin-NADP reductase in the last step of the electron chain in photosynthesis reactions. Many oxidoreductases and all ligases use NADP/NADPH as coenzyme. NADP can also be used in the determination of amylase, creatine kinase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests NADP Regenerating Buffer I 1 x 10ml NADP Regenerating Buffer II 1 x 10ml NADP Regenerating Enzyme Mix 1 vial NADPH 1 x 2 vials -
Research areas
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Relevance
NADP (Nicotinamide adenine dinucleotide phosphate) is a coenzyme composed of ribosylnicotinamide 5-phosphate (NMN) coupled by pyrophosphate linkage to the 5-phosphate adenosine 2,5-biphosphate. It serves as an electron carrier in a number of reactions, being alternately oxidised (NADP+) and reduced (NADPH). The oxidative phase of the pentose phosphate pathway is the major source of NADPH in cells, producing approxiamtely 60% of the NADPH required. NADPH provides the reducing equivalents for biosynthetic reactions and the oxidation-reduction involved in protecting against the toxicity of ROS, allowing the regeneration of GSH. NADPH is also used for anabolic pathways, such as lipid synthesis, cholesterol synthesis and fatty acid chain elongation.
