NADH Oxidase Activity Assay Kit (Fluorometric) (ab273345)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
NADH Oxidase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
NADH Oxidase Activity Assay Kit (Fluorometric) (ab273345) couples oxidation of NADH by NADH Oxidase (NOX) and reduction of a colorless probe to a brightly colored product generating fluorescence at Ex/Em = 535/587 nm. The fluorescence generated is directly proportional to the NOX activity in samples.
Lactate Standard ranges from 0-250 pmol/well.
The kit can be used to determine NOX activity in a variety of sample type with a detection limit of ~ 15 µU of NOX activity per reaction.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests L (+) Lactate Standard 1 x 100µl NADH Oxidase Assay Buffer 1 x 25ml NADH Oxidase Developer Lyophilized 2 vials NADH Oxidase Enzyme mix Lyophilized 1 vial NADH Oxidase Lysis Buffer 1 x 25ml NADH Oxidase Positive Control Lyophilized 1 vial NADH Oxidase Substrate I 1 x 20µl NADH Oxidase Substrate II 1 x 300µl NOX Probe 1 x 400µl
Images
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Example data
Example of Lactate Standard Curve.
One mole of Lactate corresponds to one mole of β-NAD+ reduced to NADH, which subsequently generates one mole of reduced substrate.
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Example dataExample data.
Reaction kinetics of recombinant NADH oxidase (Positive Control) and HepG2 cell lysate.
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Example dataExample data.
NADH oxidase activity detected in rat liver lysate (1.3μg total protein), HepG2 cell lysate (6μg total protein) and HeLa cell lysate (0.86μg total protein).