Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse/Rat Visfatin ELISA Kit (ab267799)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 167.5 pg/ml
  • Range: 390.625 pg/ml - 25000 pg/ml
  • Sample type: Cell Lysate, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Mouse/Rat Visfatin ELISA Kit
    See all Visfatin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 8 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Extract 3 5.3%

  • Sample type

    Tissue Extracts, Cell Lysate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    167.5 pg/ml

  • Range

    390.625 pg/ml - 25000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 92 84% - 98%
    Cell Lysate 91 87% - 93%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat

  • Product overview

    Mouse/Rat Visfatin ELISA Kit (ab267799) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Visfatin protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat Visfatin with 167.5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Visfatin, also known as nicotinamide phosphoribosyltransferase, is the key enzyme in the salvage pathway by which the cell converts nicotinamide to nicotinamide mononucleotide. Along with its enzymatic role, Visfatin is thought to play a pivotal role in B-cell maturation and cell signaling. This protein is highly conserved throughout Mammalia, subsequently, this kit cross-reacts with human.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of mouse and rat Visfatin standard curve in 1X Cell Extraction Buffer PTR.
    Example of mouse and rat Visfatin standard curve in 1X Cell Extraction Buffer PTR.

    The Visfatin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Visfatin in mouse and rat, cell and tissue extracts, based on a 25 µg/mL extract load.
    Interpolated concentrations of native Visfatin in mouse and rat, cell and tissue extracts, based on a 25 µg/mL extract load.

    The concentrations of Visfatin were measured in duplicate and interpolated from the Visfatin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Visfatin concentration was determined to be 12,513.20 pg/mL in Raw 264.7 cell extract, 9,298.50 pg/mL in mouse liver tissue extract, 10,138.83 pg/mL in C6 cell extract, and 13,465.27 pg/mL in rat liver tissue extract.

  • Sandwich ELISA - Mouse/Rat Visfatin ELISA Kit (ab267799)
    Sandwich ELISA - Mouse/Rat Visfatin ELISA Kit (ab267799)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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