Mouse Vitronectin ELISA kit (ab272778)
Key features and details
- Range: 0.1 ng/ml - 100 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Other biological fluids
- Detection method: Colorimetric
- Assay type: Quantitative
Overview
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Product name
Mouse Vitronectin ELISA kit
See all Vitronectin/S-Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Sample 1 20 0.45ng/ml 7.69% Sample 2 20 3.02ng/ml 4.67% Sample 3 20 6.57ng/ml 5.7% -
Sample type
Other biological fluids, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Quantitative -
Range
0.1 ng/ml - 100 ng/ml -
Assay duration
Multiple steps standard assay -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Mouse Vitronectin Primary Antibody Lyophilized Vial 1 vial Mouse Vitronectin ELISA Plate 1 unit Mouse Vitronection Standard Lyophilized Vial 1 vial Streptavidin-HRP Secondary Reagent 1 vial TMB Substrate 1 x 10ml -
Research areas
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Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
Tissue specificity
Plasma. -
Sequence similarities
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
Domain
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
Post-translational
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Complement S Protein
- Epibolin
- S Protein
see all -
Database links
- Entrez Gene: 22370 Mouse
- SwissProt: P29788 Mouse
Images
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Example data
A typical standard curve. Example only.