Mouse Uromodulin ELISA Kit (ab245726)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 212 pg/ml
- Range: 625 pg/ml - 40000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Uromodulin ELISA Kit
See all UMOD kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 4% Inter-assay Sample n Mean SD CV% serum 3 1.2% -
Sample type
Urine, Serum, Tissue Extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
212 pg/ml -
Range
625 pg/ml - 40000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 117 112% - 121% Serum 87 80% - 92% Tissue Extracts 94 92% - 96% Cell culture media 99 99% - 100% EDTA Plasma 89 87% - 91% Cit plasma 84 82% - 87% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Uromodulin ELISA Kit (ab245726) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Uromodulin protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Uromodulin with 212 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Uromodulin Capture Antibody 1 x 600µl 10X Mouse Uromodulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse Uromodulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Not known. May play a role in regulating the circulating activity of cytokines as it binds to IL-1, IL-2 and TNF with high affinity. -
Tissue specificity
Synthesized by kidney. Most abundant protein in normal human urine. -
Involvement in disease
Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1) [MIM:162000]. HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis.
Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2) [MIM:603860]. MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade.
Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI) [MIM:609886]. GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability. -
Sequence similarities
Contains 3 EGF-like domains.
Contains 1 ZP domain. -
Cellular localization
Cell membrane. Secreted. Secreted after cleavage in the urine. - Information by UniProt
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Alternative names
- ADMCKD2
- FJHN
- HNFJ
see all -
Database links
- Entrez Gene: 22242 Mouse
- SwissProt: Q91X17 Mouse
- Unigene: 10826 Mouse
Images
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Other - Mouse Uromodulin ELISA Kit (ab245726)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Uromodulin standard curve in Sample Diluent NS.The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse Uromodulin standard curve in 1X Cell Extraction Buffer PTR.The Uromodulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native Uromodulin in mouse serum, plasma samples.The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 28,874 pg/mL in serum, 21.506 pg/mL in plasma (citrate), 27,219 pg/mL in plasma (EDTA), and 39,374 pg/mL in plasma (heparin). -
Interpolated concentrations of native Uromodulin in mouse urine.The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 12,867 ng/mL in urine. -
Interpolated concentrations of spiked Uromodulin in cell culture media.The concentrations of Uromodulin were measured in duplicates, interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). -
Interpolated concentrations of native Uromodulin in mouse kidney tissue extract based on a 100 µg/mL extract load.The concentrations of Uromodulin were measured in duplicate and interpolated from the Uromodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Uromodulin concentration was determined to be 41,624 pg/mL in kidney tissue extract. -
Sandwich ELISA - Mouse Uromodulin ELISA Kit (ab245726)To learn more about the advantages of recombinant antibodies see here.
