Mouse TYRO3 ELISA Kit (DTK) (ab267577)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.4 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TYRO3 ELISA Kit (DTK)
See all TYRO3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 ng/ml -
Range
0.4 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 71.55 67% - 80% Plasma 72.51 68% - 78% Cell culture media 71.99 68% - 76% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TYRO3 ELISA Kit is designed for the quantitative determination of TYRO3 in cell culture supernatants, plasma and serum samples.
? Note: Mouse TYRO3 concentration is low in normal serum and plasma samples, and may not be detectable in this assay.
This assay employs an antibody specific for mouse TYRO3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TYRO3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TYRO3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TYRO3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- ?, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ?, IL-1?, IL-2, IL-3, IL3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1?, MIP-1?, MIP-2, MIP-3?, MIP-3?, PF-4, P-Selectin, RANTES, SCF, SDF-1?, TARC, TCA-3, TECK, TIMP-1, TNF-?, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Mouse TYRO3 coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Mouse TYRO3 Detection Antibody 2 vials Mouse TYRO3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor. -
Tissue specificity
Abundant in the brain and lower levels in other tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Brt
- BYK
- DTK
see all -
Database links
- Entrez Gene: 22174 Mouse
- SwissProt: P55144 Mouse
- Unigene: 2901 Mouse
- Unigene: 424496 Mouse
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.