Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse TIMP1 ELISA Kit (ab196265)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 6.2 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse TIMP1 ELISA Kit
    See all TIMP1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 6.7%

  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    6.2 pg/ml

  • Range

    15.6 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 111 110% - 113%
    Cell culture extracts 104 90% - 119%
    Cell culture media 107 106% - 108%
    Cit plasma 112 109% - 113%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Cow, Pig

  • Product overview

    Mouse TIMP1 ELISA Kit (ab196265) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP1 protein in cell culture supernatant, serum, plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TIMP1 with 6.2 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Tissue metalloproteinase inhibitor 1 (TIMP1or Metalloproteinase inhibitor 1) inhibits matrix metalloproteinases (MMP’s) which cause degradation of the extracellular matrix. TIMP1 inhibits MMP’s by forming a non-covalent bond complex at their catalytic zinc cofactor site and irreversibly inactivating them. TIMP1 also promotes cell proliferation and has some anti-apoptotic functions.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse TIMP1 ELISA Kit (ab196265)
    Other - Mouse TIMP1 ELISA Kit (ab196265)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of TIMP1 standard curve prepared in Sample Diluent NS.
    Example of TIMP1 standard curve prepared in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of TIMP1 in Mouse serum and plasma (citrate).
    Interpolated concentrations of TIMP1 in Mouse serum and plasma (citrate).

    The concentrations of TIMP1 were measured in duplicate and interpolated from the TIMP1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 4,495 pg/mL in Mouse serum and 4,210 pg/mL in Mouse plasma.

  • Titration of L929 Extract diluted 62.5 µg/mL to 4 µg/mL in Sample Diluent NS.
    Titration of L929 Extract diluted 62.5 µg/mL to 4 µg/mL in Sample Diluent NS.

    Background subtracted data values (mean +/- SD, n = 2) are graphed.

  • Comparison of secreted TIMP1 in unstimulated and PMA/PHA-stimulated L929 Cells.
    Comparison of secreted TIMP1 in unstimulated and PMA/PHA-stimulated L929 Cells.

    L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. TIMP1 was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the TIMP1 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 4.1 ng/mL unstimulated, 18.5 ng/mL stimulated. There was no detectable signal in media.

  • Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
    Sandwich ELISA - Mouse TIMP1 ELISA Kit (ab196265)
    To learn more about the advantages of recombinant antibodies see here.

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