Mouse Thrombomodulin ELISA Kit (ab209880)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.5 pg/ml
- Range: 93.75 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Thrombomodulin ELISA Kit
See all Thrombomodulin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% mouse serum 8 2.5% Inter-assay Sample n Mean SD CV% mouse serum 3 8.3% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
17.5 pg/ml -
Range
93.75 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 104 98% - 115% Serum 105 101% - 107% Cell culture media 94 91% - 98% Hep Plasma 92 86% - 99% EDTA Plasma 101 97% - 103% Cit plasma 102 93% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Thrombomodulin ELISA Kit (ab209880) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombomodulin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Thrombomodulin with 17.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse Thrombomodulin, which is encoded by the THBD gene, is an endothelial cell membrane protein that inhibits coagulation. Specifically, Thrombomodulin forms a 1:1 complex with thrombin, and this complex converts protein C to activated protein C. Activated protein C is an anticoagulant that inactivates coagulation factors Va and VIIIa. Human and rat Thrombomodulin show 67% and 86% amino acid sequence identity, respectively, to mouse Thrombomodulin. In humans, Thrombomodulin plays a role in thrombosis, arteriosclerosis, and cancer.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Thrombomodulin Capture Antibody 1 x 600µl 10X Mouse Thrombomodulin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse Thrombomodulin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. -
Tissue specificity
Endothelial cells are unique in synthesizing thrombomodulin. -
Involvement in disease
Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
Post-translational
modificationsN-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AHUS 6
- AHUS6
- BDCA 3
see all -
Database links
- Entrez Gene: 21824 Mouse
- SwissProt: P15306 Mouse
- Unigene: 24096 Mouse
Images
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Other - Mouse Thrombomodulin ELISA Kit (ab209880)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Thrombomodulin standard curveBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Thrombomodulin in mouse serum, plasma (citrate), plasma (EDTA), and plasma (heparin)The concentrations of Thrombomodulin were measured in duplicates, interpolated from the Thrombomodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 111.2 ng/mL in serum, 88.6 ng/mL in plasma (citrate), 95.4 ng/mL in plasma (EDTA), and 119.6 ng/mL in plasma (heparin).
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Interpolated concentrations of native Thrombomodulin in mouse urine and mouse lung supernatant samples and spiked Thrombomodulin in cell culture mediaThe concentrations of Thrombomodulin were measured in duplicates, interpolated from the Thrombomodulin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 5%, mouse lung supernatant 1:150, and cell culture media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Thrombomodulin concentration was determined to be 81.9 ng/mL in urine, 805.5 ng/mL in lung supernatant and 25.8 ng/mL in cell culture media. Mouse lung supernatant was cultured for 6 days in cell culture media plus 10% Fetal Bovine Serum and 5 μg/mL LPS.
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Sandwich ELISA - Mouse Thrombomodulin ELISA Kit (ab209880)To learn more about the advantages of recombinant antibodies see here.
