Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT) (ab137994)
Key features and details
- Sensitivity: 1.5 pg/ml
- Range: 0.031 ng/ml - 0.5 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Thrombin-Antithrombin Complexes ELISA Kit (TAT)
See all Thrombin-Antithrombin Complex kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.6% Inter-assay Sample n Mean SD CV% Overall 10.1% -
Sample type
Cell culture supernatant, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.5 pg/ml -
Range
0.031 ng/ml - 0.5 ng/ml -
Recovery
97 %
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Assay time
5h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
TAT Complexes Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TAT Complexes levels in plasma and cell culture supernatants.
A TAT Complexes specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TAT Complexes specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TAT Complexes captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse TAT Complexes Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml TAT Complexes Microplate (12 x 8 well strips) 1 unit TAT Complexes Standard 1 vial -
Research areas
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Relevance
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Cellular localization
Secreted -
Alternative names
- antithrombin III
- AT3
- AT3D
see all -
Database links
- Entrez Gene: 11905 Mouse
- Entrez Gene: 14061 Mouse
- SwissProt: P32261 Mouse
- SwissProt: P19221 Mouse
Images
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ELISA: ab137994 Thrombin-Antithrombin Complexes (TAT) Mouse ELISA Kit
Mouse TAT measured in murine biological fluids (diluted 1/256; duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab137994.