Mouse soluble IL-2 Receptor ELISA Kit (ab277724)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse soluble IL-2 Receptor ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.7% Inter-assay Sample n Mean SD CV% Serum 3 3.6% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15 pg/ml -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105 101% - 107% Serum 98 79% - 112% Cell culture media 120 119% - 122% Hep Plasma 104 104% - 105% EDTA Plasma 91 79% - 107% Cit plasma 97 86% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Human NFAT2 ELISA kit (ab277724) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NFAT2 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NFAT2 with 4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-2 Receptor is a plasma membrane receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. Interleukin-2 receptor is a non-covalent dimer of an alpha and a beta subunit. Interleukin-2 receptor exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. This kit is designed to detect the soluble form of Interleukin-2 receptor subunit alpha (soluble IL-2 Receptor).
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse soluble IL-2 Receptor Capture Antibody 1 x 600µl 10X Mouse soluble IL-2 Receptor Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse soluble IL-2 Receptor Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
The IL-2 Receptor, which is involved in T cell-mediated immune responses, alpha (IL-2RA) and beta (IL-2RB) chains, together with the common gamma chain (IL-2RG), constitute the high-affinity IL-2 receptor. Homodimeric alpha chains (IL-2RA) result in low-affinity receptor. Normally an integral-membrane protein, soluble IL-2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL-2RA mRNAs have been isolated, but the significance of each is presently unknown. Homodimeric beta (IL-2RB) chains produce a medium-affinity receptor. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The interleukin 2 (IL-2) receptor gamma chain (IL-2RG), an important signalling component of many interleukin receptors (IL-2,IL-4,IL-7,IL-9, and IL-15), is thus referred to as the common gamma chain. Mutations in this X-chromosome-linked gene cause X-linked severe combined immunodeficiency (XSCID). -
Cellular localization
Cell Membrane; single-pass type I membrane protein. -
Alternative names
- CD122
- CD122 antigen
- CD132
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse soluble IL-2 Receptor standard curve in Sample Diluent NS.The soluble IL-2 Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native soluble IL-2 Receptor in mouse serum, plasma and spleen supernatant samples.The concentrations of soluble IL-2 Receptor were measured in duplicates, interpolated from the soluble IL-2 Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25% and spleen supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean soluble IL-2 Receptor concentration was determined to be 7,908 pg/mL in neat serum, 7,484 pg/mL in neat plasma (citrate), 5,442 pg/mL in neat plasma (EDTA), 7,172 pg/mL in neat plasma (heparin) and 837 pg/mL in neat spleen supernatant.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
