Mouse S100A8 ELISA Kit (ab263886)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.97 pg/ml
- Range: 15.625 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse S100A8 ELISA Kit
See all MRP8 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 3.9% Inter-assay Sample n Mean SD CV% Plasma 3 3.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.97 pg/ml -
Range
15.625 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 114 104% - 119% Serum 106 102% - 109% Hep Plasma 108 105% - 113% EDTA Plasma 100 92% - 105% Cit plasma 102 96% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse S100A8 ELISA Kit (ab263886) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of S100A8 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse S100A8 with 7.97 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
S100A8 is a protein also known as calgranulin A that is encoded by the S100A8 gene. Moreover, it is a calcium and zinc binding protein, which serves a vital role in the regulation of inflammatory process and immune response. Also involved in a wide variety of intra and extracellular functions such as cell cycle progression and differentiation. Studies have shown altered expression of S100A8 to be associated with cystic fibrosis. S100A8 is known to bind with S100A9 in which forms a heterodimer called calprotectin.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse S100A8 Capture Antibody 1 x 600µl 10X Mouse S100A8 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse S100A8 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
Tissue specificity
Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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Alternative names
- 60B8Ag
- AI323541
- B8Ag
see all -
Database links
- Entrez Gene: 20201 Mouse
- SwissProt: P27005 Mouse
- Unigene: 21567 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse S100A8 standard curve in Sample Diluent NS.The S100A8 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native S100A8 in mouse serum, plasma and tissue culture supernatant samples.The concentrations of S100A8 were measured in duplicates, interpolated from the S100A8 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 5%, plasma EDTA 5%, plasma (heparin) 25% and lung supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean S100A8 concentration was determined to be 1,163 pg/mL in serum, 5,005 pg/mL in plasma (citrate) and 9,576 pg/mL in plasma (EDTA), 920.1 pg/mL in plasma (heparin), and 9,214 pg/mL in lung supernatant.
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Sandwich ELISA - Mouse S100A8 ELISA Kit (ab263886)To learn more about the advantages of recombinant antibodies see here.
