Mouse RGM-C ELISA Kit (ab243685)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16.3 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse RGM-C ELISA Kit
See all Hemojuvelin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 3.7% Inter-assay Sample n Mean SD CV% serum 3 5.8% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16.3 pg/ml -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 109 106% - 112% Cell culture media 91 85% - 94% Hep Plasma 98 84% - 115% EDTA Plasma 111 106% - 115% Cit plasma 108 101% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse RGM-C ELISA Kit (ab243685) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RGM-C protein in cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse RGM-C with 16.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse RGM-C Capture Antibody 1 x 600µl 10X Mouse RGM-C Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse RGM-C Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Mutations in Repulsive Guidance Molecule (also known as RGM-C; HJV; hemojuvelin; Hfe2) cause juvenile hemochromatosis, a severe iron overload disease. RGM-C gene expression has been characterized in the developing mouse and found to be exclusively expressed in all striated muscle and in the myocardium. -
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Alternative names
- 2310035L15Rik
- 5230400G09Rik
- AI414844
see all -
Database links
- Entrez Gene: 69585 Mouse
- SwissProt: Q7TQ32 Mouse
Images
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Other - Mouse RGM-C ELISA Kit (ab243685)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse RGM-C Standard Curve Comparison.Standard Curve comparison between mouse RGM-C SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse RGM-C standard curve in Sample Diluent NS.The RGM-C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native RGM-C in mouse serum and plasma samples.The concentrations of RGM-C were measured in duplicates, interpolated from the RGM-C standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasm (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RGM-C concentration was determined to be 7,101.4 pg/mL in serum, 4,286.1 pg/mL in plasma (citrate), 4,373.6 pg/mL in plasma (EDTA), and 6,311.1 pg/mL in plasma (heparin).
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Interpolated concentrations of spike RGM-C in cell culture media.The concentrations of RGM-C were measured in duplicates, interpolated from the RGM-C standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Mouse RGM-C ELISA Kit (ab243685)To learn more about the advantages of recombinant antibodies see here.
