Mouse PP2A-alpha ELISA Kit (ab209878)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 66.2 pg/ml
- Range: 85.9 pg/ml - 5500 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PP2A-alpha ELISA Kit
See all PP2A-alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell extract 8 5.5% Inter-assay Sample n Mean SD CV% Cell extract 5 7.3% -
Sample type
Cell culture extracts, Tissue Extracts, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
66.2 pg/ml -
Range
85.9 pg/ml - 5500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 102 98% - 106% Tissue Extracts 97 92% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PP2A-alpha ELISA Kit (ab209878) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PP2A-alpha protein in tissue extracts, tissue homogenate, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PP2A-alpha with 66.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
PP2A-alpha is a highly conserved heterotrimeric phosphatase for microtubule-associated proteins. Additionally, PP2A-alpha targets include proteins in oncogenic signalling cascades, including AKT, MEK and Raf.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse PP2A-alpha Capture Antibody 1 x 600µl 10X Mouse PP2A-alpha Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse PP2A-alpha Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-1 subfamily. -
Post-translational
modificationsReversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
Cellular localization
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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Alternative names
- PP2A alpha
- PP2A-alpha
- PP2AA_HUMAN
see all -
Database links
- Entrez Gene: 19052 Mouse
- SwissProt: P63330 Mouse
- Unigene: 260288 Mouse
Images
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Other - Mouse PP2A-alpha ELISA Kit (ab209878)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse PP2A-alpha standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PP2A in mouse NIH-3T3 extract based on a 12 µg/mL extract load and mouse skeletal muscle extract based on a 20 µg/mL extract load.The concentrations of PP2A-alpha were measured in duplicate and interpolated from the PP2A-alpha standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PP2A-alpha concentration was determined to be 4,273 pg/mL in NIH-3T3 extract and 2,719 pg/mL in mouse skeletal muscle extract.
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This assay detects human and rat PP2A-alpha.The concentrations of PP2A-alpha in PC12 and HeLa extract were measured in duplicate, interpolated from the mouse PP2A-alpha standard curves and corrected for sample dilution. Undiluted samples are PC12 and HeLa extract based on a 10 μg/mL extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PP2A-alpha concentration was determined to be 4,956 pg/mL in PC12 extract and 4,395 pg/mL in HeLa extract.
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Sandwich ELISA - Mouse PP2A-alpha ELISA Kit (ab209878)To learn more about the advantages of recombinant antibodies see here.
