Mouse PLGF ELISA Kit (ab197748)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.526 pg/ml
- Range: 4.69 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PLGF ELISA Kit
See all PLGF kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.27% Inter-assay Sample n Mean SD CV% Serum 3 6.5% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.526 pg/ml -
Range
4.69 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 103.8 101.3% - 107.1% Cell culture media 98.2 96.2% - 100.6% Cit plasma 110.7 100.6% - 118.8% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Goat, Cow, Pig -
Product overview
Mouse PLGF ELISA Kit (ab197748) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PLGF protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PLGF with 0.526 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Placental growth factor (PLGF) is an angiogenic factor that is variably glycosylated and secreted. The PLGF protein is a 55-60 kDa disulfide linked homodimer and it belongs to the small family of PDGF/VEGF-related angiogenic growth factors, all of which contain 8 conserved cysteines. Due to alternative splicing, there are three human PLGF mRNAs encoding human PLGF-1, -2, and -3 isoform precursors. There is a unique 21 aa insert at the C-terminus of the PLGF-2 isoform and this isoform is the heparin binding form of PLGF. In mouse tissues there is currently only one identified PLGF mRNA form encoding the equivalent of human PLGF-2. Mouse PLGF is a 158 aa polypeptide that contains an 18 aa signal sequence and a 140 aa mature segment. Human and rat PLGF share 66% and 91% aa sequence identity, respectively, to mouse PLGF.
Homodimeric mouse PLGF binds the tyrosine kinase receptor VEGF R1/Flt-1. This receptor is a 180 kDa type I transmembrane glycoprotein with high affinity for PLGF. In addition to forming homodimers, PLGF also forms heterodimers with VEGF-A isoforms, forming bioactive molecules. When heterodimerized with VEGF, PLGF will activate VEGF R2.
A limited number of functions have been directly attributed to PLGF. It is known to induce monocyte activation and to provide a stimulus for monocyte migration. PLGF also upregulates VEGF production in PBMCs and induces tissue factor secretion in both monocytes and HUVECs. Furthermore, PLGF is also reported to serve as a growth and chemotactic factor for endothelial cells. Finally, PLGF is known to be expressed by trophoblasts and may protect against growth factor withdrawal-induced trophoblast apoptosis.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse PLGF Capture Antibody 1 x 600µl 10X Mouse PLGF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Mouse PLGF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent 75BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. -
Tissue specificity
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
Isoform PlGF-2 contains a basic insert which acts as a cell retention signal. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. - Information by UniProt
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Alternative names
- D12S1900
- Pgf
- PGFL
see all -
Database links
- Entrez Gene: 18654 Mouse
- SwissProt: P49764 Mouse
- Unigene: 4809 Mouse
Images
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Other - Mouse PLGF ELISA Kit (ab197748)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse PLGF standard curve comparision dataStandard curve comparison between mouse PLGF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 2-fold sensitivity.
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Example of PLGF mouse standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of PLGF mouse standard curve in Sample Diluent 75BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of PLGF mouse standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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PLGF Mouse expression is shown for cultured media from three Mouse cell lines.L929, J774A.1 and RAW264.7 cells were cultured in HGDMEM with 10% fetal calf serum or Horse serum, 2mM L-glutamine and 100 µg/mL Kanamycin. L929 and J774A.1 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10ng/mL of PMA. RAW264.7 cells were starved for 24 hours and treated in the presence and absence of 5 µg/mL of LPS. Samples were loaded on the plate undiluted and the concentrations of PLGF were interpolated from a calibration curve diluted in NS. The mean PLGF concentration was determined to be 178.9 pg/mL in stimulated L929 cells, 15.8 pg/mL in unstimulated L929 cells, 32.8 pg/mL in stimulated J774A.1 cells, 0.4 pg/mL in unstimulated J774A.1 cells, 4.4 pg/mL in stimulated RAW264.7 cells, and 2.3 pg/mL in unstimulated RAW264.7 cells.
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Sandwich ELISA - Mouse PLGF ELISA Kit (ab197748)To learn more about the advantages of recombinant antibodies see here.
