Mouse PD1 ELISA Kit (ab210971)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PD1 ELISA Kit
See all PD1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% EL-4 ext 5 4% Inter-assay Sample n Mean SD CV% EL-4 ext 3 7% -
Sample type
Cell culture supernatant, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
21 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 93% - 104% Cell culture extracts 109 100% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PD1 ELISA Kit (ab210971) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD1 protein in cell culture extracts, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD1 with 21 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
PD1 is a single-pass type I cell membrane glycoprotein. It is a receptor for PD-1L and/or PD-L2 ligands. PD1 is involved in the regulation of T-cell function during immunity and tolerance. PD-1 interactions with its ligands PD-L1 and/or PD-L2 provides a negative regulatory signal to CD4 and CD8 T cells that results ultimately in a phenotype termed T cell exhaustion. Possible cell death inducer, in association with other factors.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse PD-1 Capture Antibody 1 x 600µl 10X Mouse PD-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Denaturant 1 x 500µl Mouse PD-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Database links
- Entrez Gene: 18566 Mouse
- SwissProt: Q02242 Mouse
- Unigene: 5024 Mouse
Images
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Other - Mouse PD1 ELISA Kit (ab210971)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse PD1 standard curve 1X Cell Extraction Buffer PTR+Enhancer.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse PD1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PD1 in mouse EL-4 cell extract samples based on a 90 µg/mL extract load.The concentrations of PD1 were measured in duplicate and interpolated from the PD1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 4,246 pg/mL in EL-4 cell extract.
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Interpolated concentrations of native PD1 in PHA/PMA treated Day 2 EL-4 cell culture supernatant samples.The concentrations of PD1 were measured in duplicates, interpolated from the PD1 standard curves and corrected for sample dilution. Undiluted samples are as follows: EL-4 cell culture supernatant 100%. Dilutions beyond 4 fold were not analyzed due to O.D. values below the O.D. values of the MDD. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 195 pg/mL in EL-4 cell culture supernatant samples.
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Comparison of PD1 in unstimulated and PHA/PMA (1.5% and 10ng/mL respectively) stimulated EL-4 supernatant.EL-4 cells were grown in the absence or presence of PHA/PMA for 48 hours. The concentrations of PD1 were measured in neat supernatant samples in duplicates and interpolated from the PD1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean PD1 concentration was determined to be 32 pg/mL in unstimulated, 203 pg/ml in PHA/PMA stimulated supernatants and undetectable in media (not shown).
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Sandwich ELISA - Mouse PD1 ELISA Kit (ab210971)To learn more about the advantages of recombinant antibodies see here.
