Mouse MSP ELISA Kit (ab256390)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 129.22 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Lysate, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MSP ELISA Kit
See all Macrophage Stimulating Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 1.8% Inter-assay Sample n Mean SD CV% Serum 3 2.3% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Tissue Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
129.22 pg/ml -
Range
312.5 pg/ml - 20000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 93% - 103% Urine 100 99% - 100% Serum 110 109% - 110% Hep Plasma 105 107% - 107% EDTA Plasma 109 103% - 120% Cit plasma 111 110% - 111% Tissue Lysate 108 106% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MSP ELISA Kit (ab256390) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MSP protein in cit plasma, edta plasma, hep plasma, serum, urine, tissue lysate, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MSP with 129.22 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Macrophage stimulating protein (MSP) is a serum protein also known as hepatocyte growth factor-like that is encoded by the MST1 gene. Secreted by the liver as the main source, MSP has the ability to exert biological effects such as proliferation, migration, differentiation, and phagocytosis through binding to the receptor tyrosine kinase. The standard protein in this kit is a fragment of the full length protein that contains the alpha chain.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MSP Capture Antibody 1 x 600µl 10X Mouse MSP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse MSP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Macrophage Stimulating Protein contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. -
Alternative names
- D3F15S2
- D3F15S2h
- D9H3F15S2
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse MSP standard curve in Sample Diluent NS.The MSP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MSP in mouse serum and plasma samples.The concentrations of MSP were measured in duplicates, interpolated from the MSP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:400, plasma (citrate) 1:200, plasma (EDTA) 1:400 and plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MSP concentration was determined to be 6,320 ng/mL in serum, 1,580 pg/mL in plasma (citrate), 4,435 pg/mL in plasma (EDTA), and 4,262 pg/mL in plasma (heparin).
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Interpolated concentrations of native MSP in mouse urine and tissue liver supernatant samples.The concentrations of MSP were measured in duplicates, interpolated from the MSP standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100% and liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MSP concentration was determined to be 1,895 pg/mL in urine and 37,547 pg/mL in liver supernatant.
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Interpolated concentrations of native MSP in mouse tissue liver extract based on a 250 µg/mL extract load.The concentrations of MSP were measured in duplicate and interpolated from the MSP standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MSP concentration was determined to be 7,300 pg/mL.
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Sandwich ELISA - Mouse MSP ELISA Kit (ab256390)To learn more about the advantages of recombinant antibodies see here.
