Mouse LIX ELISA Kit (CXCL5) (ab264611)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.14 pg/ml
- Range: 7.03 pg/ml - 450 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse LIX ELISA Kit (CXCL5)
See all CXCL5 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.5% Inter-assay Sample n Mean SD CV% Serum 3 9.6% -
Sample type
Cell culture supernatant, Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.14 pg/ml -
Range
7.03 pg/ml - 450 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 96 91% - 103% Urine 88 87% - 89% Serum 93 80% - 110% Tissue Extracts 96 83% - 111% Hep Plasma 100 94% - 115% EDTA Plasma 101 91% - 109% Cit plasma 88 83% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse LIX ELISA Kit (CXCL5) (ab264611) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LIX (CXCL5) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, urine, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LIX (CXCL5) with 1.14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Mouse LIX (LPS-induced CXC chemokine) also known as CXCL5, is a member of the ELR+ group of 7-9 kDa neutrophil and monocyte chemotactic proteins in the CXCL cytokine family. Mouse LIX can be produced by fibroblasts, epithelial cells (including alveolar type II epithelia), endothelial cells, platelets, cardiac myocytes, preosteoblasts, oligodendrocytes, and adipose-resident macrophages. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. Endotoxemia increases LIX expression, especially in the heart, but also in the lung, spleen, and liver. LIX is downregulated by glucocorticoids and is considered a glucocorticoid-attenuated response gene or GARG. It can also be downregulated by IL-10 and viral proteins. LIX is the most potent mouse neutrophil chemoattractant and activator LIX participates in the induction of LPS-induced acute lung injury and in lung ischemia-induced angiogenesis. High expression of LIX in obese white adipose tissue is thought to contribute to insulin resistance.
Mouse CXCL5 is the equivalent of human CXCL6 based on genetic and biological evidence as pointed out by Nomiyama et al. (PMID: 20434943).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse LIX (CXCL5) Capture Antibody 1 x 600µl 10X Mouse LIX (CXCL5) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse LIX (CXCL5) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2(1-78) and, more potent, GCP-2(9-78) attract neutrophils and are involved in neutrophil activation. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsGCP-2(1-78) and GCP-2(9-78) are produced by proteolytic cleavage after secretion from fibroblasts and epithelial cells. GCP-2(9-78) is the most prominent form. A number of additional N-terminal (processed between pos. 41 and 48) and C-terminal (processed between pos. 118 and 132) processed forms have been identified, probably also representing intermediate states. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- C-X-C motif chemokine 5
- Cxcl5
- CXCL5_MOUSE
see all -
Database links
- Entrez Gene: 20311 Mouse
- SwissProt: P50228 Mouse
- Unigene: 4660 Mouse
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Mouse LIX (CXCL5) standard curve comparisonStandard Curve comparison between Mouse LIX (CXCL5) SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
-
Example of mouse LIX standard curve in Sample Diluent NS. The LIX standard curve was prepared as described in Section 10.The LIX standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native LIX in mouse serum, and plasma samples.The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (citrate) 10%, plasma (EDTA) 10%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 20.21 ng/mL in serum, 1.37 ng/mL in plasma (citrate), 3.49 ng/mL in plasma (EDTA), and 5.04 ng/mL in plasma (heparin).
-
Interpolated concentrations of native LIX in mouse LPS stimulated lung culture supernatant and LPS stimulated spleen culture supernatant samples.The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows: LPS stimulated lung culture supernatant 1% and LPS stimulated spleen culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 43.12 ng/mL in LPS stimulated lung supernatant and 1.25 ng/mL in LPS stimulated spleen supernatant.
-
Interpolated concentrations of native LIX in mouse lung, spleen, and bone marrow based on a 500 µg/mL extract load.The concentrations of LIX were measured in duplicate and interpolated from the LIX standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 571.73 pg/mL in lung extract, 548.73 pg/mL in spleen extract and 353.24 pg/mL in bone marrow extract.
-
Interpolated concentrations of native LIX in stimulated vs unstimulated mouse tissue culture supernatant samples.The concentrations of LIX were measured in duplicates, interpolated from the LIX standard curves and corrected for sample dilution. Undiluted samples are as follows: lung supernatant 100%, LPS stimulated lung supernatant 1%, spleen supernatant 100%, and LPS stimulated spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LIX concentration was determined to be 177.56 pg/mL in lung supernatant, 43.12 ng/mL LPS stimulated lung supernatant, 23.65 pg/mL spleen supernatant and 1250 pg/mL LPS stimulated spleen supernatant.
-
Sandwich ELISA - Mouse LIX ELISA Kit (CXCL5) (ab264611)To learn more about the advantages of recombinant antibodies see here.
