Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CXCL15 antibody [EPR14470] (ab197016)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14470] to CXCL15
Suitable for: IHC-P, IP, WB
Reacts with: Mouse

Product name

Anti-CXCL15 antibody [EPR14470]
See all CXCL15 primary antibodies
Description

Rabbit monoclonal [EPR14470] to CXCL15
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IP: Mouse lung lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14470
Isotype

IgG
Research areas

Western blot - Anti-CXCL15 antibody [EPR14470] (ab197016)

All lanes : Anti-CXCL15 antibody [EPR14470] (ab197016) at 1/5000 dilution

Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse heart tissue lysate
Lane 4 : Mouse kidney tissue lysate
Lane 5 : Mouse spleen tissue lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 19 kDa
Observed band size: 19 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] (ab197016)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] (ab197016)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] (ab197016)

CXCL15 was immunoprecipitated from Mouse lung lysates with ab197016 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197016 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Mouse lung lysate 10 µg (Input). Lane 2: ab197016 IP in Mouse lung lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197016 in Mouse lung lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-CXCL15 antibody [EPR14470] (ab197016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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