Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL15 antibody [EPR14470] (ab197016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14470] to CXCL15
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CXCL15 antibody [EPR14470]
    See all CXCL15 primary antibodies

  • Description

    Rabbit monoclonal [EPR14470] to CXCL15

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IP: Mouse lung lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14470

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CXCL15 antibody [EPR14470] (ab197016)
    Western blot - Anti-CXCL15 antibody [EPR14470] (ab197016)
    All lanes : Anti-CXCL15 antibody [EPR14470] (ab197016) at 1/5000 dilution

    Lane 1 : Mouse lung tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Mouse kidney tissue lysate
    Lane 5 : Mouse spleen tissue lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] (ab197016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] (ab197016)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] (ab197016)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL15 antibody [EPR14470] (ab197016)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CXCL15 with ab197016 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on mouse kidney tissue. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] (ab197016)
    Immunoprecipitation - Anti-CXCL15 antibody [EPR14470] (ab197016)

    CXCL15 was immunoprecipitated from Mouse lung lysates with ab197016 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab197016 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse lung lysate 10 µg (Input). Lane 2: ab197016 IP in Mouse lung lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197016 in Mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-CXCL15 antibody [EPR14470] (ab197016)
    Anti-CXCL15 antibody [EPR14470] (ab197016)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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