Mouse Interferon gamma ELISA Set (without plates) (ab47618)
Overview
-
Product name
Mouse Interferon gamma ELISA Set (without plates)
See all Interferon gamma kits -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s IFNγ Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IFNγ in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IFN-gamma ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IFN-gamma in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine Interferon gamma Range: 31.25 pg/ml - 1000 pg/ml Sensitivity
-
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-Murine IFN gamma antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Murine IFN gamma Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml IFN gamma Standard (Lyophilized) Yellow 5 vials 10 vials 15 vials Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials -
Research areas
-
Function
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
Tissue specificity
Released primarily from activated T lymphocytes. -
Involvement in disease
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
Sequence similarities
Belongs to the type II (or gamma) interferon family. -
Post-translational
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- IF 1
- IFG
- IFI
see all -
Database links
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
Images
-
Murine Interferon gamma ELISA Set (ab47618)Murine IFN-gamma Standard curve ranging from 31.25 to 1000 pg/mL
