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Mouse IL-20 ELISA Kit (ab235645)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 7 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse IL-20 ELISA Kit
    See all IL-20 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.4%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    7 pg/ml

  • Range

    15.6 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 105 95% - 120%
    Cell culture media 101 92% - 111%
    Hep Plasma 99 91% - 114%
    EDTA Plasma 88 79% - 103%
    Cit plasma 108 99% - 115%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse IL-20 ELISA Kit (ab235645) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-20 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-20 with 7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse IL-20 Capture Antibody 1 x 600µl
    10X Mouse IL-20 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse IL-20 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.

  • Tissue specificity

    Expressed at very low levels in skin, trachea, and other tissues.

  • Sequence similarities

    Belongs to the IL-10 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Cytokine Zcyto10
    • Four alpha helix cytokine
    • IL-20
    • IL10D
    • IL20
    • IL20_HUMAN
    • Interleukin 20
    • Interleukin-20
    • MGC96907
    • UNQ852/PRO1801
    • ZCYTO10
    see all

  • Database links

    Images

    • Other - Mouse IL-20 ELISA Kit (ab235645)
      Other - Mouse IL-20 ELISA Kit (ab235645)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Mouse IL-20 Standard Curve Comparison
      Mouse IL-20 Standard Curve Comparison

      Standard Curve comparison between Mouse IL-20 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

    • Interpolated concentrations of native IL-20 in mouse serum and plasma samples.
      Interpolated concentrations of native IL-20 in mouse serum and plasma samples.

      The concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (citrate) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-20 concentration was determined to be 221 pg/mL in serum, 212 pg/mL in plasma (EDTA), 207 pg/mL in plasma (citrate), and 206 pg/mL in plasma (heparin).

    • Interpolated concentrations of spike IL-20 in Mouse cell culture media sample.
      Interpolated concentrations of spike IL-20 in Mouse cell culture media sample.

      The concentrations of IL-20 were measured in duplicate and interpolated from the IL-20 standard curve and corrected for sample dilution.

    • Example of mouse IL-20 standard curve in Sample Diluent NS.
      Example of mouse IL-20 standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Sandwich ELISA - Mouse IL-20 ELISA Kit (ab235645)
      Sandwich ELISA - Mouse IL-20 ELISA Kit (ab235645)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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