Mouse IL-1a Matched Antibody Pair Kit (ab210893)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Mouse IL-1a Matched Antibody Pair Kit
See all IL-1 alpha kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.33 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-1a Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-1a.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab199076. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse IL-1a Capture Antibody 2 x 50µg 1 x 50µg Mouse IL-1a Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse IL-1a Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
Sequence similarities
Belongs to the IL-1 family. -
Domain
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
Cellular localization
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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Alternative names
- BAF
- FAF
- Hematopoietin 1
see all -
Database links
- Entrez Gene: 16175 Mouse
- SwissProt: P01582 Mouse
- Unigene: 15534 Mouse
Images
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Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse IL-1a Matched Antibody Pair Kit (ab210893)To learn more about the advantages of recombinant antibodies see here.
