Mouse IL-12 p70 ELISA Kit (ab119531)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-12 p70 ELISA Kit
See all IL-12 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 7 8.3% Inter-assay Sample n Mean SD CV% Overall 7 11% -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
106 %
Sample specific recovery Sample type Average % Range Serum 106 95% - 118% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
IL-20 p70 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-20 p70 in serum and cell culture supernatants.
IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-mouse IL-12 p70 monoclonal antibody 1 x 70µl Microplate coated with anti-mouse IL-12 p70 monoclonal antibody (12 x 8 wells) 1 unit Mouse IL-12 p70 Standard, lyophilized (2 ng/mL on reconstitution). 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CLMF
- CLMF p35
- CLMF p40
see all -
Database links
- Entrez Gene: 16159 Mouse
- Entrez Gene: 16160 Mouse
- SwissProt: P43431 Mouse
- SwissProt: P43432 Mouse
- Unigene: 103783 Mouse
Images
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Typical Standard Curve
Representative Standard Curve using ab119531.