Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)
![Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454748-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrymolt4human1.jpg "Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)")
Overview
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Product name
Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free -
Tested applications
Suitable for: Flow Cyt, ICCmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
ab280757 is the carrier-free version of ab280756. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280757 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
R313-MouseIgG2c -
Isotype
IgG2c
Images
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Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)
This image was produced using ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in MOLT-4 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454747-antimouseigg2cantibodyr313mouseigg2cflowcytometrymolt4human.jpg)
Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454746-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrynih3t3mouse1.jpg)
Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in NIH/3T3 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454745-antimouseigg2cantibodyr313mouseigg2cflowcytometrynih3t3mouse.jpg)
Flow Cytometry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast, Left) / PC-12 (Rat adrenal gland pheochromocytoma, Right) cells labelling Mouse IgG2c with ab280756 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse IgG2c, kappa monoclonal [18C8BC7AD10] - Isotype Control (ab170191, Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
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![Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454744-antimouseigg2cantibodyr313mouseigg2cimmunocytochemistrypc12rat1.jpg)
Immunocytochemistry - Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)This image was produced using ab280756, the same clone but in a different formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NPC-12 cells labelling Mouse IgG2c with ab280756 at 1/80 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing no staining in PC-12 cells.
Negative control 1: ab280756 at a 1/80 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)](https://www.abcam.com/ps/products/280/ab280757/Images/ab280757-454743-benefitsofrecombinantantibodies.png)
Mouse IgG2c monoclonal [R313-MouseIgG2c]-Isotype control - BSA and Azide free (ab280757)
