Mouse IgG ELISA kit - 30 minutes (ab273150)
Key features and details
- Sensitivity: 6 ng/ml
- Range: 20 ng/ml - 1900 ng/ml
- Sample type: Ascites Fluid, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
Overview
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Product name
Mouse IgG ELISA kit - 30 minutes
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A 1/100 9 10.63µg/ml 5.33 % B 1/100 9 11.56µg/ml 5.33 % C 1/100 9 22.61pg/ml 8.48 % D 1/100 9 28.88µg/ml 10.03 % E 1/100 9 66.82µg/ml 8.39 % F 1/100 9 75.92µg/ml 9.9 % G 1/100 9 102.47µg/ml 10.38 % Inter-assay Sample n Mean SD CV% H 1/250 30 3.45 % H 1/500 30 2.99 % H 1/1000 30 4.97 % -
Sample type
Cell culture supernatant, Ascites Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
6 ng/ml -
Range
20 ng/ml - 1900 ng/ml -
Assay duration
One step assay -
Product overview
The Mouse IgG quantification Kit (ab273150) provides a rapid and easy method (one step ELISA) for the quantitative determination of mouse IgG in cell culture supernatant and ascitic fluid.
The kit includes ready-to-use reagents necessary to analyze up to 90 samples in 30 min. Buffer solutions are color coded in order to simplify pipetting steps.
A polyclonal antibody specific to mouse IgG (H+L) is pre-coated onto microwells. Samples and standards are pipetted into microwells and mouse IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of mouse IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10 x 96 tests Detection antibody 1 x 12ml 1 x 120ml Mouse IgG standard 0.0 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 0.02 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 0.1 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 0.2 µg/ml 6 x 0.3ml 6 x 1ml Mouse IgG standard 0.75 µg/ml 1 x 0.3ml 1 x 1ml Mouse IgG standard 1.9 µg/ml 1 x 0.3ml 1 x 1ml Pre-coated 8 microwells strips 1 x 96 tests 10 x 96 tests Sample Diluent 1 x 30ml 1 x 500ml Stop Solution 1 x 12ml 1 x 60ml TMB Substrate 1 x 12ml 1 x 60ml -
Research areas
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Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
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Images
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Mouse Immunoglobulin Quantification Standard curve
Example of Mouse IgG quantitative kit Standard curve
