Cyclic GMP ELISA Kit (ab133026)
Key features and details
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
Overview
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Product name
Cyclic GMP ELISA Kit
See all cGMP kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Plasma -
Assay type
Competitive -
Assay time
3h 0m -
Assay duration
Multiple steps standard assay -
Product overview
Abcam’s Cyclic GMP (cGMP) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cyclic GMP in saliva, plasma (Heparin, EDTA), urine, and serum and tissue culture supernatants.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-cGMP antigen and a polyclonal rabbit antibody specific to cGMP. After incubation the excess reagents are washed away. pNpp substrate is added. After a short incubation the alkaline phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of cGMP captured in the plate.
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Notes
Guanosine 3’, 5’-cyclic monophosphate (cyclic GMP; cGMP) has been shown to be present at levels typically 10-100 fold lower than cAMP in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. It is involved in a number of important biological reactions. Some hormones, such as acetylcholine, insulin and oxytocin, as well as certain other chemicals like serotonin and histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate and nitric oxide (NO) also stimulate cGMP levels. Peptides, such as atrial natriuretic peptide (ANP) that relax smooth muscle also increase cGMP concentrations. cGMP has been confirmed as a second messenger for ANP. NO can be synthesized from L-arginine and diffuse through cell membranes. The interaction of NO with guanylate cyclase allows cGMP to act as a third messenger in some cells.
Cross Reactivity
Compound % Cross Reactivity cGMP 100 GMP GTP cAMP AMP ATP cUMP CTP -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Acetylation kit - Acetic Anhydride 1 x 1ml Acetylation kit - Triethylamine 1 x 2ml Assay Buffer 2 1 x 27ml Cyclic GMP Complete Alkaline Phosphatase Conjugate 1 x 5ml Cyclic GMP Complete Antibody 1 x 5ml Cyclic GMP Complete Standard 1 x 500µl Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
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Relevance
Cyclic guanosine monophosphate (cGMP) serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG). -
Alternative names
- Cyclic GMP
- Cyclic guanosine monophosphate
- Guanosine 3 5 Cyclic Monophosphate
Images
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Typical Standard Curve
Representative Standard Curve using ab133026.
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Typical Standard CurveRepresentative Standard Curve using ab133026.
