Mouse Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229431)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 12 pg/ml
- Range: 19.53 pg/ml - 40000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Heme Oxygenase 1 ELISA Kit, Fluorescent
See all Heme Oxygenase 1 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Cell medium 5 2.4% Inter-assay Sample n Mean SD CV% Cell medium 3 2.6% -
Sample type
Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
12 pg/ml -
Range
19.53 pg/ml - 40000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 105 102% - 111% Serum 96 94% - 98% Tissue Extracts 110 104% - 113% Cell culture media 112 108% - 119% Hep Plasma 88 86% - 91% EDTA Plasma 89 87% - 91% Cit plasma 95 87% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow, Pig -
Product overview
Heme Oxygenase 1 (HO 1) in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Heme Oxygenase 1 (HO 1) protein in mouse serum, plasma, urine, cell culture supernatant, and cell and tissue extract samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint® SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
Heme oxygenase 1 (HO 1) is an enzyme that functions in heme catabolism. The activity of heme oxygenase 1 is to cleave the heme ring to form biliverdin. There are two isozymes of heme oxygenase that have 47% amino acid sequence identity: inducible heme oxygenase 1 and constitutively expressed heme oxygenase 2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Mouse HO 1 Capture Antibody 1 x 600µl 10X Mouse HO 1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Mouse HO 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
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Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
Sequence similarities
Belongs to the heme oxygenase family. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
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Alternative names
- 32 kD
- bK286B10
- D8Wsu38e
see all -
Database links
- Entrez Gene: 15368 Mouse
- SwissProt: P14901 Mouse
- Unigene: 276389 Mouse
Images
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Other - Mouse Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229431)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Heme Oxygenase 1 (HO 1) standard curve in Sample Diluent NS. -
Interpolated concentrations of Heme Oxygenase 1 in mouse serum, and splenocyte supernatant.The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 10,670 pg/mL in mouse serum and 6,084 pg/mL in mouse splenocyte supernatant.
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Interpolated concentrations of Heme Oxygenase 1 in mouse plasma (EDTA), plasma (heparin) and plasma (citrate).The concentrations of Heme Oxygenase 1 were measured in duplicate and interpolated from the Heme Oxygenase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Heme Oxygenase 1 concentration was determined to be 5,536 pg/mL in mouse plasma (EDTA), 6,400 pg/mL in mouse plasma (heparin), and 5,479 pg/mL in mouse plasma (Citrate).
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Quantitation of Heme Oxygenase 1 expression in different cell extracts.Interpolated values of Heme Oxygenase 1 are plotted for the indicated cell extracts (y-axis) against the total extract load (x-axis).
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Sandwich ELISA - Mouse Heme Oxygenase 1 ELISA Kit, Fluorescent (ab229431)To learn more about the advantages of recombinant antibodies see here.
