Mouse GRO gamma ELISA Kit (CXCL3) (ab272191)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.3 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse GRO gamma ELISA Kit (CXCL3)
See all GRO gamma kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 9.3% Inter-assay Sample n Mean SD CV% Serum 3 1.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.3 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 97% - 103% Serum 101 93% - 108% Hep Plasma 94 88% - 101% EDTA Plasma 94 86% - 93% Cit plasma 87 84% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse GRO gamma ELISA kit (ab272191) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GRO gamma protein in serum, plasmas and tissue culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GRO gamma with 1.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Chemokines are a group of small structurally related molecules that play fundamental roles in the development, homeostasis, and function of the immune system. GRO gamma (CXCL3) is an 8 kDa proinflammatory member of the chemokine heparin-binding CXC subfamily, or alpha chemokines, and is a ligand for CXCR2. GRO gamma has chemotactic activity for neutrophils and may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Mouse GRO gamma shares 62%, 90% and 63% sequence homology with human, rat and cow, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse GRO gamma (CXCL3) Capture Antibody 1 x 600µl 10X Mouse GRO gamma (CXCL3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse GRO gamma (CXCL3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 3
- C-X-C motif chemokine ligand 3
- Chemokine (C X C motif) ligand 3
see all -
Database links
- Entrez Gene: 330122 Mouse
- SwissProt: Q6W5C0 Mouse
- Unigene: 244289 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse GRO gamma standard curve comparisonStandard Curve comparison between the original Mouse GRO gamma SimpleStep ELISA kit (ab206310) and current Mouse GRO gamma SimpleStep ELISA kit (ab272191).
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Example of mouse GRO gamma standard curve in Sample Diluent NS.The GRO gamma standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native GRO gamma in mouse serum, plasma and cell culture supernatant samples.The concentrations of GRO gamma were measured in duplicates, interpolated from the GRO gamma standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 50%, plasma EDTA (100%) and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GRO gamma concentration was determined to be 145.55 pg/mL in serum, 41.66 pg/mL in plasma (citrate), 31.17 pg/ml in plasma (EDTA) and 50.92 pg/mL in plasma (heparin).
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Interpolated concentrations of native GRO gamma in mouse lung culture supernatant and spleen culture supernatant.The concentrations of GRO gamma were measured in duplicate and interpolated from the GRO gamma standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GRO gamma concentration was determined to be 125 ng/mL in mouse lung supernatant treated with 5mg/ml LPS for 5 days and 18 ng/mL in mouse lung supernatants, mock treatment and 45.25 ng/ml in mouse spleen supernatant treated with 5mg/ml LPS for 5 days. *GRO gamma levels were assayed two times and found to be below the level of detection in mock treated mouse spleen supernatant.
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Sandwich ELISA - Mouse GRO gamma ELISA Kit (CXCL3) (ab272191)To learn more about the advantages of recombinant antibodies see here.
