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Mouse Gremlin 1 ELISA Kit (ab267578)

Key features and details

  • Sensitivity: 0.8 ng/ml
  • Range: 0.819 ng/ml - 200 ng/ml
  • Sample type: Cell culture supernatant, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Gremlin 1 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.8 ng/ml

  • Range

    0.819 ng/ml - 200 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 74.8 69% - 79%
    Plasma 86.2 80% - 93%
    Cell culture media 144.9 137% - 149%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse Gremlin 1 ELISA Kit is designed for the quantitative determination of Gremlin 1 in cell culture supernatants, plasma and serum samples.


    Δ Note: Mouse Gremlin 1 concentration is low in normal serum and plasma samples and may not be detectable in this assay.


    Δ Note: Collect plasma using EDTA as an anticoagulant; Heparin and citrate are not recommended in this assay.


    This assay employs an antibody specific for mouse Gremlin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Gremlin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Gremlin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Gremlin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine, Activin A, ADAMTS1, Adiponectin, ANG-3, ANGPTL3, Artemin, CCL28, CD36, Chordin, CRP, E-Cadherin, Epigen, Epiregulin, Fas, Galectin-7, gp130, Granzyme B, IFN-gamma R1, IL-17B, IL-17B R, IL-22, MIP-1 beta, MMP-2, MMP-3, MMP-10, PDGF-AA, Persephin, sFRP-3, Shh-N, SLAM, TCK-1, TECK, TGF beta-1, TRANCE, TremL1, TWEAK, VEGF-B, VEGF-R2.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Anti-Mouse Gremlin 1 coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Anti-Mouse Gremlin 1 Detection Antibody 2 vials
    Mouse Gremlin 1 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis.

  • Tissue specificity

    Highly expressed in small intestine, fetal brain and colon. Expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. In subjects with HMPS1, by contrast, GREM1 is expressed, not only in basal ISEMFs, but also at very high levels in epithelial cells (predominantly colonocytes), with expression extending most of the way up the sides of the crypt. Weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts.

  • Involvement in disease

    Polyposis syndrome, mixed hereditary 1

  • Sequence similarities

    Belongs to the DAN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • BMP antagonist 1
    • Cell proliferation inducing gene 2 protein
    • Cell proliferation-inducing gene 2 protein
    • CKTSF1B1
    • colorectal adenoma and carcinoma 1
    • Cysteine knot superfamily 1
    • Cysteine knot superfamily 1, BMP antagonist 1
    • Cysteine knot superfamily BMP antagonist 1
    • DAN domain family member 2
    • DAND2
    • Down regulated in Mos-transformed cells protein
    • Down-regulated in Mos-transformed cells protein
    • DRM
    • grem1
    • GREM1_HUMAN
    • GREMLIN
    • Gremlin 1 homolog, cysteine knot superfamily
    • Gremlin 1 like protein
    • Gremlin 1, cysteine knot superfamily, homolog
    • Gremlin-1
    • Gremlin1
    • IHG-2
    • IHG2
    • Increased in high glucose 2
    • Increased in high glucose protein 2
    • PIG2
    • Proliferation inducing gene 2
    • proliferation inducing gene 2 protein
    see all

  • Database links

    Images

    • Mouse Gremlin 1 ELISA kit example standard curve
      Mouse Gremlin 1 ELISA kit example standard curve

      This standard curve is for demonstration only. A standard curve must be run with each assay.

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