Mouse Flt3 ligand ELISA Kit (Flt3L) (ab275551)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.9 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Flt3 ligand ELISA Kit (Flt3L)
See all Flt3 / CD135 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.4% Inter-assay Sample n Mean SD CV% Serum 3 12.4% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.9 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 84% - 103% Cell culture media 101 94% - 105% Hep Plasma 93 92% - 95% EDTA Plasma 97 91% - 100% Cit plasma 89 81% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse FLT3 Ligand ELISA kit (ab275551) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse FLT3 Ligand protein in serum, plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FLT3 Ligand with 3.9 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Flt-3 ligand is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 ligand consists of a 158 amino acid extracellular domain with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Flt-3 ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Both transmembrane and soluble Flt-3 ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. Animal studies report Flt-3 ligand to reduce the severity of experimentally induced allergic inflammation. Human Flt-3 ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Flt3 ligand (Flt3L) Capture Antibody 1 x 600µl 10X Mouse Flt3 ligand (Flt3L) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse Flt3 ligand (Flt3L) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for the FL cytokine. Has a tyrosine-protein kinase activity. -
Tissue specificity
Bone marrow cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD 135
- CD135
- CD135 antigen
see all -
Database links
- Entrez Gene: 14255 Mouse
- SwissProt: Q00342 Mouse
- Unigene: 194 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse Flt3 ligand competitor curve comparisonStandard Curve comparison between Mouse Flt3 ligand SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse Flt3 ligand standard curve in Sample Diluent NS.The Flt3 ligand standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Flt3 ligand in mouse serum and plasma samples.The concentrations of Flt3 ligand were measured in duplicates, interpolated from the Flt3 ligand standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Flt3 ligand concentration was determined to be 232.95 pg/mL in serum, 163.28 pg/mL in plasma (citrate), 184.73 pg/mL in plasma (EDTA)and 269.77 pg/mL in plasma (heparin).
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Sandwich ELISA - Mouse Flt3 ligand ELISA Kit (Flt3L) (ab275551)To learn more about the advantages of recombinant antibodies see here.
