Mouse Factor D ELISA Kit (ab170244)
Key features and details
- Sensitivity: 0.03 ng/ml
- Range: 0.031 ng/ml - 1 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Factor D ELISA Kit
See all Factor D/CFD kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.1% Inter-assay Sample n Mean SD CV% Overall 9.3% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.03 ng/ml -
Range
0.031 ng/ml - 1 ng/ml -
Recovery
97 %
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Assay time
4h 00m -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s Factor D Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor D in cell culture supernatants, urine, plasma and serum.
A Factor D specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor D specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor D captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Biotinylated Mouse Factor D Antibody 1 x 60µl 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml Chromogen Substrate 1 x 7ml Factor D Microplate (12 x 8 well strips) 1 unit Factor D Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. -
Involvement in disease
Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipsin
- Adipsin/complement factor D
- Adn
see all -
Database links
- Entrez Gene: 11537 Mouse
- SwissProt: P03953 Mouse
- Unigene: 4407 Mouse
Images
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Typical Standard Curve
Representative Standard Curve using ab170244