Mouse Eph receptor A1/EphA1 Matched Antibody Pair Kit (ab218171)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Mouse Eph receptor A1/EphA1 Matched Antibody Pair Kit
See all Eph receptor A1/EphA1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
30.4 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Eph receptor A1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Eph receptor A1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210573. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse EphA1 Capture Antibody 2 x 50µg 1 x 50µg Mouse EphA1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse EphA1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. -
Tissue specificity
Overexpressed in several carcinomas. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsPhosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- EPH
- EPH receptor A1
- EPH tyrosine kinase
see all -
Database links
- Entrez Gene: 13835 Mouse
- SwissProt: Q60750 Mouse
- Unigene: 133330 Mouse
Images
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Mouse EphA1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Eph receptor A1/EphA1 Matched Antibody Pair Kit (ab218171)To learn more about the advantages of recombinant antibodies see here.
