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Mouse EMMPRIN ELISA Kit (CD147) (ab205575)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.7 pg/ml
  • Range: 15.625 pg/ml - 1000 pg/ml
  • Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse EMMPRIN ELISA Kit (CD147)
    See all CD147 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.5%

  • Sample type

    Urine, Serum, Plasma, Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.7 pg/ml

  • Range

    15.625 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 112.4 95.8% - 128.1%
    Serum 89.9 86.1% - 93.6%
    Cell culture media 96 92.4% - 98.1%
    Hep Plasma 112.3 103.3% - 121.3%
    EDTA Plasma 84 81.1% - 88.4%
    Cit plasma 83.7 80.6% - 87.4%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Cow, Pig

  • Product overview

    Mouse EMMPRIN ELISA Kit (CD147) (ab205575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EMMPRIN (CD147) protein in plasma, serum, tissue extracts, urine, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse EMMPRIN (CD147) with 1.7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    EMMPRIN plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. EMMPRIN plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. EMMPRIN stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). EMMPRIN seems to be a receptor for oligomannosidic glycans. In vitro, EMMPRIN promotes outgrowth of astrocytic processes.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse EMMPRIN ELISA Kit (CD147) (ab205575)
    Other - Mouse EMMPRIN ELISA Kit (CD147) (ab205575)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse EMMPRIN standard curve in Sample Diluent NS.
    Example of mouse EMMPRIN standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of mouse EMMPRIN standard curve in Sample Diluent 25BS.
    Example of mouse EMMPRIN standard curve in Sample Diluent 25BS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of mouse EMMPRIN standard curve in 1X Cell extraction Buffer PTR.
    Example of mouse EMMPRIN standard curve in 1X Cell extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of EMMPRIN in mouse serum, plasma, cell culture supernatant and urine samples.
    Interpolated concentrations of EMMPRIN in mouse serum, plasma, cell culture supernatant and urine samples.

    EMMPRIN in mouse serum, plasma, cell culture supernatant and urine samples. The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Note that 1X Diluted serum and plasma samples were pre-diluted to 10%. Note that 1X Diluted Raw 264.7 day 3 cell culture supernatant samples were neat. Note that 1X Diluted Mouse Urine (Female) samples were pre-diluted to 25%. The interpolated, dilution factor-corrected values are plotted in pg of EMMPRIN per mL of neat sample (mean +/- SD, n=2).

  • Interpolated concentrations of EMMPRIN in mouse extract samples.
    Interpolated concentrations of EMMPRIN in mouse extract samples.

    The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Note that 1X Diluted Raw 264.7 cell extract samples were at 5 µg/mL. Note that 1X Diluted Small Intestine extract samples were at 500 µg/mL. The interpolated, dilution factor-corrected values are plotted in pg of EMMPRIN per µg of total protein (mean +/- SD, n=2).

  • Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (ab205575)
    Sandwich ELISA - Mouse EMMPRIN ELISA Kit (CD147) (ab205575)
    To learn more about the advantages of recombinant antibodies see here.

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