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Mouse CD14 ELISA Kit (ab242238)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 39.1 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse CD14 ELISA Kit
    See all CD14 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    plasma 5 4.3%
    Inter-assay
    Sample n Mean SD CV%
    plasma 3 3.8%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    39.1 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112 107% - 117%
    Serum 104 95% - 103%
    Hep Plasma 112 108% - 115%
    EDTA Plasma 97 94% - 98%
    Cit plasma 103 100% - 107%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse CD14 ELISA Kit (ab242238) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD14 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD14 with 39.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse CD14 ELISA Kit (ab242238)
    Other - Mouse CD14 ELISA Kit (ab242238)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Mouse CD14 Standard Curve Comparison.
    Mouse CD14 Standard Curve Comparison.
    Standard Curve comparison between mouse CD14 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
  • Example of mouse CD14 standard curve in Sample Diluent NS.
    Example of mouse CD14 standard curve in Sample Diluent NS.
    The CD14 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
  • Interpolated concentrations of native CD14 in mouse serum and plasma samples.
    Interpolated concentrations of native CD14 in mouse serum and plasma samples.
    The concentrations of CD14 were measured in duplicates, interpolated from the CD14 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:40, plasma (citrate) 1: 40, plasma (heparin) 1: 25, and plasma (EDTA) 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD14 concentration was determined to be 58.2 ng/mL in serum, 69.6 ng/mL in plasma (citrate), 65.5 ng/ml in plasma (heparin), and 173.2 ng/ml in plasma (EDTA).
  • Interpolated concentrations of native CD14 in mouse spleen tissue supernatant samples, both unstimulated and stimulated for three days with 50 ng/ml PMA and 500 ng/ml calcium ionomycin.
    Interpolated concentrations of native CD14 in mouse spleen tissue supernatant samples, both unstimulated and stimulated for three days with 50 ng/ml PMA and 500 ng/ml calcium ionomycin.
    The concentrations of CD14 were measured in duplicates, interpolated from the CD14 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant unstimulated 25% and spleen supernatant stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD14 concentration was determined to be 9.9 ng/mL in unstimulated and 16.0 ng/mL in stimulated mouse spleen supernatant.
  • Sandwich ELISA - Mouse CD14 ELISA Kit (ab242238)
    Sandwich ELISA - Mouse CD14 ELISA Kit (ab242238)
    To learn more about the advantages of recombinant antibodies see here.

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