Mouse CD105 ELISA Kit (ab240677)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 26.1 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CD105 ELISA Kit
See all CD105 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.8% Inter-assay Sample n Mean SD CV% Serum 3 5.1% -
Sample type
Cell culture supernatant, Urine, Serum, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26.1 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 102% - 118% Urine 90 89% - 91% Serum 116 103% - 133% Tissue Extracts 84 71% - 96% Cell culture media 108 103% - 117% EDTA Plasma 115 108% - 125% Cit plasma 103 97% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CD105 ELISA Kit (ab240677) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD105 protein in cell culture supernatant, cit plasma, edta plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD105 with 26.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CD105 Capture Antibody 1 x 600µl 10X Mouse CD105 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse CD105 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AI528660
- AI662476
- CD 105
see all -
Database links
- Entrez Gene: 13805 Mouse
- SwissProt: Q63961 Mouse
- Unigene: 225297 Mouse
Images
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Other - Mouse Endoglin ELISA Kit (ab240677)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse Endoglin Standard Curve Comparison.Standard Curve comparison between mouse Endoglin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of mouse Endoglin standard curve in Sample Diluent NS.The Endoglin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse Endoglin standard curve in 1X Cell Extraction Buffer PTR.The Endoglin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native Endoglin in mouse serum and plasma samples.The concentrations of Endoglin were measured in duplicates, interpolated from the Endoglin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endoglin concentration was determined to be 16.17 ng/mL in neat serum, 10.98 ng/mL in neat plasma (citrate), and 11.89 ng/mL in plasma (EDTA). -
Interpolated concentrations of native Endoglin in mouse urine and spleen tissue supernatant (2 days) samples.The concentrations of Endoglin were measured in duplicates, interpolated from the Endoglin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%, and spleen tissue supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endoglin concentration was determined to be 1.22 ng/mL in neat urine and 3.39 ng/mL in neat spleen tissue supernatant. -
Interpolated concentrations of native Endoglin in mouse spleen tissue extract based on a 100 µg/mL extract load.The concentrations of Endoglin were measured in duplicate and interpolated from the Endoglin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endoglin concentration was determined to be 3.00 ng/mL in mouse spleen tissue extract. -
Sandwich ELISA - Mouse CD105 ELISA Kit (ab240677)To learn more about the advantages of recombinant antibodies see here.