Mouse alpha-1-antitrypsin ELISA Kit (ab267809)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 65 ng/ml
- Range: 104.7 ng/ml - 6700 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse alpha-1-antitrypsin ELISA Kit
See all alpha 1 Antitrypsin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.7% Inter-assay Sample n Mean SD CV% Serum 4 4.7% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
65 ng/ml -
Range
104.7 ng/ml - 6700 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 113 108% - 117% Cell culture media 90 88% - 93% Hep Plasma 108 103% - 112% EDTA Plasma 102 86% - 116% Cit plasma 111 104% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse alpha-1-antitrypsin ELISA Kit (ab267809) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha-1-antitrypsin protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse alpha-1-antitrypsin with 65 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse Alpha-1-antitrypsin is an inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse alpha-1-antitrypsin Capture Antibody 1 x 600µl 10X Mouse alpha-1-antitrypsin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse alpha-1-antitrypsin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
Tissue specificity
Ubiquitous. Expressed in leukocytes and plasma. -
Involvement in disease
Alpha-1-antitrypsin deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Post-translational
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
Cellular localization
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Alternative names
- A1A
- A1AT
- A1AT_HUMAN
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse alpha-1-antitrypsin standard curve in Sample Diluent NS.The alpha-1-antitrypsin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native alpha-1-antitrypsin in mouse serum, plasma (EDTA), plasma (citrate) and plasma (heparin) samples.The concentrations of alpha-1-antitrypsin were measured in duplicates, interpolated from the alpha-1-antitrypsin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (EDTA) 1:133, plasma (citrate), and plasma (heparin) 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean alpha-1-antitrypsin concentration was determined to be 210 µg/mL in serum, 416 µg/mL in plasma (EDTA), 149 µg/mL in plasma (citrate), and 221 µg/mL in plasma (heparin).
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Sandwich ELISA - Mouse alpha-1-antitrypsin ELISA Kit (ab267809)To learn more about the advantages of recombinant antibodies see here.
