Mitochondrial Staining Reagent - Orange 405 - CytoPainter (ab176834)
Overview
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Product name
Mitochondrial Staining Reagent - Orange 405 - CytoPainter
See all Mitochondria kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Mitochondrial Staining Reagent - Orange 405 | CytoPainter (ab176834) is part of a series of new fluorogenic probes to label mitochondria of live cells. The proprietary mitochondria dye selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells and becomes trapped in mitochondria after it gets into cells. The fluorescent mitochondrial indicator is retained in mitochondria for a long time since it carries a cell-retaining group. This key feature significantly increases the staining efficiency; make it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.
MitoOrange 405 Indicator Reagent is well excited by the violet laser at Ex=405 nm, thus is particularly useful for microscopes and flow cytometers with a violet laser source.
The dye will stain live cells but is not well-retained after fixation.
Review other dyes and kits for mitochondrial staining, or the live cell staining fluorescent dyes guide
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Notes
Mitochondria are membrane-enclosed organelles found in most eukaryotic cells. Mitochondria are sometimes described as “cellular power plants” because they generate most of the cellular supply of ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders and cardiac dysfunction, and may play a role in the aging process. Although most of a cellular DNA is contained in the cell nucleus, the mitochondrion has its own independent genome.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests CytoPainter MitoOrange 405 Indicator Reagent 1 x 500 tests -
Research areas
Images
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HeLa cells stained with CytoPainter MitoOrange 405 Indicator Reagent (ab176834)
Image of HeLa cells stained with Abcam's CytoPainter MitoOrange 405 Indicator Reagent (ab176834) in a Costar black wall/clear bottom 96-well plate.
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Spectrum of Abcam's CytoPainter MitoOrange 405 Indicator Reagent (ab176834).Spectrum of CytoPainter MitoOrange 405 Indicator Reagent (ab176834).