Anti-MAL antibody (ab15418)
Key features and details
- Rabbit polyclonal to MAL
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Xenopus laevis
- Isotype: IgG
Overview
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Product name
Anti-MAL antibody
See all MAL primary antibodies -
Description
Rabbit polyclonal to MAL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human MAL aa 114-126.
Sequence:CFDGFTY(R/K)HYHENI
(Peptide available asab39812)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAL antibody (ab15418)All lanes : Anti-MAL antibody (ab15418) at 1/5000 dilution
Lane 1 : Mouse mpkCCD cell lysate
Lane 2 : Xenopus 2f3 (Xenopus laevis) cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit
Predicted band size: 18 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAL antibody (ab15418)IHC image of ab15418 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab15418, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
